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. 2019 Sep 9;11(9):1336. doi: 10.3390/cancers11091336

Figure 5.

Figure 5

Piper genus-derived compounds induce cell death. (A) terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of MCF7 cells treated for 12 h in the absence (CTRL, control) and in the presence of gibbilimbol B/eriopodol A (30 µg/mL) or erioquinol (10 µg/mL). 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) was used for nuclei detection. Scale bar = 50 µm. (B) Bright field microscopy of MCF7 cells treated for 6 h in the absence (CTRL) and in the presence of gibbilimbol B, eriopodol A, or erioquinol at increasing concentrations. Scale bar = 100 µm. (C) 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) staining of MCF7 cells treated for 6 h in the absence (CTRL) and in the presence of gibbilimbol B/eriopodol A (30 µg/mL) or erioquinol (10 µg/mL). Scale bar = 10 µm. Lower panels represent enlarged image details. (D) Evaluation by flow cytometry of Annexin V-fluorescein isothiocyanate(FITC)/propidium iodide (PI) staining in MCF7 cells treated in the absence (CTRL) and in the presence of 10 µg/mL erioquinol, for 3 and 6 h. Quadrants are drawn, and relative proportion of labelled cells is indicated. The events shown in the lower left-hand quadrant are unlabeled cells. Images and data are representative of four independent experiments.