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. 2019 Sep 9;11(9):1336. doi: 10.3390/cancers11091336

Figure 6.

Figure 6

Piper genus-derived compounds induce cell death. Western blot analysis of cleaved-caspase 9 and 7 in MCF7 cells treated for increasing times in the absence (CTRL, control) and in the presence of (A) gibbilimbol B or (B) eriopodol A. Vinculin was used as internal standard. Right panels: densitometric analysis expressed as fold change of CTRL. Images and data are representative of three-five independent experiments. * p < 0.01, ** p < 0.001, and *** p < 0.0001 relative to CTRL. (C) Western blot analysis of cleaved-caspase 7 in MCF7 cells treated for increasing times in the absence (CTRL) and in the presence of 10 µg/mL erioquinol. The stain-free gel was used as loading control. Images are representative of three independent experiments. PC: positive control. (D) Immunofluorescence imaging of cleaved-caspase 7 (punctate red pattern) in MCF7 cells treated for 3 and 6 h in the absence (CTRL) and in the presence of gibbilimbol B/eriopodol A (30 µg/mL) or erioquinol (10 µg/mL). 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (blue) and phalloidin (green) were used for nuclei and cytoskeleton detection, respectively. Images are representative of four independent experiments. Scale bar = 25 µm.