Figure 9.

Confocal microscopy for reactive oxygen species (ROS) detection. (A) MCF7 and (B) U373 cells were treated for 6 h in the absence (CTRL, control) and in the presence of gibbilimbol B/eriopodol A (30 µg/mL) or erioquinol (10 µg/mL). Cells were then stained for ROS (2’-7’dichlorofluorescin diacetate - DCFH-DA, green). 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (blue) and phalloidin (red) were used for nuclei and cytoskeleton detection, respectively. The images are representative of three independent experiments. Scale bar: 25 µm.