Table 2.
Gene expression analysis by nanocapillary qRT-PCR of 624 genes (TaqMan® OpenArray® Human Cancer Panel, QuantStudio™ 12K Flex) of A549 xenografts (in vivo) compared to 2D monolayer (Petri-dish) cultures resulted in the selection of sixty genes: 29 upregulated (pink) and 31 downregulated (green) for further studies. Significance (p) was calculated by Student’s t-test as pairwise comparison of ΔCt values.
| Gene Symbol | Assay ID | ΔΔCt (log2) | SD | Significance (p) | Gene Symbol | Assay ID | ΔΔCt (log2) | SD | Significance (p) |
|---|---|---|---|---|---|---|---|---|---|
| CEACAM5 | Hs00944025_m1 | 9.11 | 1.77 | 0.0220 | JUN | Hs00277190_s1 | −2.34 | 1.97 | 0.2740 |
| APPL1 | Hs00179382_m1 | 8.17 | 0.12 | 0.0001 | MYC | Hs99999003_m1 | −2.42 | 1.96 | 0.2306 |
| LCN2 | Hs01008571_m1 | 5.58 | 0.68 | 0.0082 | MKI67 | Hs01032443_m1 | −2.43 | 0.45 | 0.0249 |
| SEPP1 | Hs01032845_m1 | 5.26 | 1.10 | 0.0293 | FTL | Hs00830226_gH | −2.51 | 1.21 | 0.1293 |
| PRDX2 | Hs03044902_g1 | 4.71 | 1.45 | 0.0590 | CTPS | Hs00157163_m1 | −2.53 | 1.41 | 0.1831 |
| TGFBR1 | Hs00610318_m1 | 4.38 | 0.02 | 0.0001 | E2F1 | Hs00153451_m1 | −2.63 | 1.58 | 0.1555 |
| CP | Hs00236810_m1 | 4.36 | 0.08 | 0.1173 | PFKP | Hs00242993_m1 | −2.64 | 1.70 | 0.1746 |
| ANPEP | Hs00952642_m1 | 3.79 | 2.19 | 0.2148 | FBN2 | Hs00266592_m1 | −2.65 | 0.17 | 0.2083 |
| DLG3 | Hs00221664_m1 | 3.72 | 0.22 | 0.0213 | CYR61 | Hs00155479_m1 | −2.79 | 2.20 | 0.2390 |
| CA9 | Hs00154208_m1 | 3.71 | 1.42 | 0.0886 | CTSL2 | Hs00822401_m1 | −2.81 | 1.03 | 0.0769 |
| CD24 | Hs00273561_s1 | 3.66 | 0.54 | 0.0182 | EGFR | Hs01076078_m1 | −2.82 | 0.38 | 0.0345 |
| IFITM1 | Hs00705137_s1 | 3.60 | 0.25 | 0.0057 | IGFBP4 | Hs00181767_m1 | −2.83 | 1.73 | 0.1601 |
| PECAM1 | Hs00169777_m1 | 3.54 | 0.00 | 0.0217 | FGFR1 | Hs00241111_m1 | −2.87 | 1.74 | 0.2655 |
| MX1 | Hs00895608_m1 | 3.53 | 0.92 | 0.0702 | AXL | Hs01064444_m1 | −2.87 | 1.81 | 0.1685 |
| TMEM45A | Hs01046616_m1 | 3.49 | 1.42 | 0.2538 | ASNS | Hs00370265_m1 | −2.98 | 1.02 | 0.0890 |
| KRT19 | Hs00761767_s1 | 3.38 | 1.35 | 0.0793 | SOCS3 | Hs02330328_s1 | −3.04 | 1.01 | 0.1962 |
| TLR3 | Hs00152933_m1 | 3.37 | 0.48 | 0.0330 | IER3 | Hs00174674_m1 | −3.18 | 1.78 | 0.2419 |
| ERBB3 | Hs00176538_m1 | 3.27 | 0.95 | 0.0524 | CDC25B | Hs01550934_m1 | −3.20 | 1.51 | 0.1116 |
| IGFBP5 | Hs01052296_m1 | 3.18 | 1.03 | 0.0563 | BAX | Hs00180269_m1 | −3.32 | 1.22 | 0.0804 |
| CDKN1B | Hs00153277_m1 | 2.91 | 0.22 | 0.0217 | TFAP2C | Hs00231476_m1 | −3.60 | 1.58 | 0.0921 |
| SFN | Hs00968567_s1 | 2.83 | 0.61 | 0.0329 | ABCG2 | Hs01053790_m1 | −3.66 | 0.85 | 0.0368 |
| HYAL1 | Hs00201046_m1 | 2.74 | 0.38 | 0.0096 | NR4A1 | Hs00374226_m1 | −3.66 | 0.83 | 0.0250 |
| MYB | Hs00920554_m1 | 2.54 | 1.61 | 0.2887 | RAB6B | Hs00981572_m1 | −3.66 | 0.89 | 0.0446 |
| PDGFB | Hs00966522_m1 | 2.47 | 0.47 | 0.0853 | CD70 | Hs00174297_m1 | −3.94 | 0.00 | 0.1385 |
| ERBB2 | Hs01001580_m1 | 2.40 | 1.25 | 0.1180 | CSF1 | Hs00174164_m1 | −4.03 | 0.19 | 0.0107 |
| GRB7 | Hs00918009_g1 | 2.39 | 0.67 | 0.3032 | FYN | Hs00176628_m1 | −4.09 | 1.86 | 0.0988 |
| FN1 | Hs01549976_m1 | 2.38 | 1.25 | 0.1188 | PLCG2 | Hs00182192_m1 | −4.13 | 2.19 | 0.1465 |
| CEBPG | Hs00156454_m1 | 2.37 | 1.99 | 0.5677 | EGR1 | Hs00152928_m1 | −4.19 | 2.60 | 0.1582 |
| IGFBP3 | Hs00181211_m1 | 2.34 | 1.03 | 0.1114 | ID1 | Hs03676575_s1 | −4.38 | 0.22 | 0.0155 |
| ADM | Hs00181605_m1 | −4.51 | 0.10 | 0.0013 | |||||
| FOS | Hs00170630_m1 | −4.61 | 2.40 | 0.1254 |