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. 2019 Sep 7;11(9):1328. doi: 10.3390/cancers11091328

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Confocal images confirm the A23187- and Ionomycin-induced NETosis suppression by anthracyclines. Fluorescence staining and confocal microscopy of neutrophils was performed on 5 μM samples of each anthracycline with and without A23187 and ionomycin. Neutrophils were stained for DNA (blue) and MPO (green); colocalization of these stains shows NETs being released in samples without anthracyclines. With anthracyclines present, the neutrophil nuclei are more condensed and significantly fewer NETs release (n = 3; scale bar, 20 μm).