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. 2019 Sep 7;11(9):1328. doi: 10.3390/cancers11091328

Figure 7.

Figure 7

Dexrazoxane does not induce NETosis, nor alter the effect of anthracyclines on NETosis and anthracycline-mediated NETosis suppression does not induce apoptosis. NETosis assays were performed on human neutrophils that were plated with one concentration (5 μM) of epirubicin, daunorubicin, doxorubicin, or idarubicin, dexrazoxane (0.0, 1.0, 2.5, 5.0 μM) and 5 μM Sytox Green fluorescence dye. NETosis was induced using either media (-ve control), PMA (25 nM), or A23187 (4 μM). Fluorescence intensities (proxy for DNA release) were measured by a plate reader every 30 min for 4 h. % DNA release for each condition compared to Triton –X-100 sample (100% DNA release) was calculated. Bar charts were created to compare % DNA release. For (A), dexrazoxane alone did not affect the production of NETs as % DNA release is similar to control conditions. For (B,C), it is shown that dexrazoxane did not affect how anthracyclines suppress NETosis via either pathway (n = 3, * p < 0.05; One-way ANOVA with Tukey’s post-test compared to 0.0 μM dosage of control drug control).