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. 2019 Sep 7;11(9):1328. doi: 10.3390/cancers11091328

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Anthracycline-mediated NETosis suppression does not induce apoptosis. Neutrophils were incubated with anthracyclines and then induced to produce NETs using either media only, PMA (25 nM) or A23187 (4 μM) and then fixed to 8-chamber slides. cCasp-3 and DNA were stained with anti-cleaved cCasp-3 rabbit primary antibody/goat anti-rabbit secondary antibody (red) and DAPI (blue), respectively. For –ve control (media only), PMA, and A23187 conditions, cCasp-3 release was not increased compared to media control (n = 3; scale bar 20 μM). The counting data of NETosis and apoptosis is represented in Figure S4.