Figure 4.

The amounts of ED-1 and IL-1β remain high in rats treated with AngII for 4 weeks. Immunostaining for ED-1 were performed in renal samples (A). Representative pictures of at least three different kidneys and four different fields are shown. Scale bar = 100 μm. Brown staining represents ED-1 positive immunoreactivity. The staining is indicated by black arrows. The semi-quantitative morphometric analysis for ED-1 was performed as explained in the Materials and Methods. Renal homogenates were used to measure IL-1β as marker of inflammation (B). The bars represent the means ± SE of a n ≥ 4 rats per experimental group. The differences between the subgroups of each of the three groups were evaluated by an ANOVA followed by a Tuckey test. ** p < 0.01 and * p < 0.05 vs. AngII groups; && p < 0.01 vs. AngII 3 + 2 and AngII 4 + 2.