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. 2019 Sep 9;20(18):4437. doi: 10.3390/ijms20184437

Figure 1.

Figure 1

Expression profiles of OsWRKY5 in rice. (a) OsWRKY5 mRNA levels in detached organs from the japonica cultivar ‘Dongjin’ (hereafter wild type; WT) at the heading stage. OsWRKY5 was mainly expressed in leaf blade and leaf sheath. (b,c) Changes in OsWRKY5 expression level in leaf blades of WT rice grown in a paddy field (b) or in the greenhouse (c) under natural long day conditions (≥14 h light/day). (c) Detached leaves of three-week-old WT were incubated in 3 Mm 2-(N-morpholino)ethanesulfonic (MES) buffer (pH 5.8) at 28 °C in complete darkness. Red arrow indicates heading date. (d) Expression of OsWRKY5 measured in flag leaves divided into four regions from the green sector (a) to the yellow sector (d) at 128 days after transplanting (DAT). OsWRKY5 mRNA levels were determined by RT-qPCR analysis and normalized to that of OsUBQ5 (Os01g22490). Mean and SD values were obtained from at least three biological samples. Experiments were repeated twice with similar results. DDI, day(s) of dark incubation.