Figure 3.

OsWRKY5 promotes leaf yellowing under dark-induced senescence (DIS) conditions. WT and mutant lines (oswrky5-D and oswrky5) were grown in paddy soil for four weeks under natural long day conditions (≥14 h light/day). (a,b) Yellowing of detached leaves induced in 3 mM MES buffer (pH 5.8) at 28 °C under complete darkness. Changes in leaf color (a,b) and total chlorophyll (Chl) contents (c,d) of oswrky5-D or oswrky5 mutants compared with the WT after 4 or 5 days of dark incubation (DDI), respectively. Mean and SD values were obtained from more than three biological replicates. Asterisks indicate a statistically significant difference from WT, as determined by Student’s t-test (*** p < 0.001). Experiments were repeated twice with similar results. FW, fresh weight.