Figure 4.

Altered expression of CDGs and SAGs in oswrky5-D and oswrky5 during DIS. Total RNA was isolated from detached leaves of WT and mutant lines (oswrky5-D and oswrky5) under DIS as shown in Figure 3 (3a,b). Expression of CDGs and SAGs in oswrky5-D (a–f) or oswrky5 (g–l) was compared with that in the WT after 4 or 5 DDI, respectively. Transcript levels of CDGs (a–c and g–i) and SAGs (d–f and j–l) were determined by RT-qPCR analysis and normalized to that of OsUBQ5. Mean and SD values were obtained from more than three biological replicates. Asterisks indicate a statistically significant difference from WT, as determined by Student’s t-test (* p < 0.05, ** p < 0.01, *** p < 0.001). Experiments were repeated twice with similar results. CDGs, Chl degradation genes; DDI, day(s) of dark incubation; SAGs, senescence-associated genes.