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. 2019 Sep 7;9(9):457. doi: 10.3390/biom9090457

Figure 1.

Figure 1

2’-O-methylation guide activity of Drosophila scaRNA:MeU2-C28 and mutants thereof, detected in injected Xenopus oocytes. (A) Postulated base-pairing of scaRNA:MeU2-C28 with U2 snRNA. (B) Schematic representation of tested MeU2-C28 variants. Experimentally verified guide activity or lack thereof are indicated with a plus or minus sign for each variant. Mutants that lacked the CAB box behaved like the corresponding full-length variants, thus confirming that the CAB box is dispensable for modification guide activity.