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. 2019 Sep 7;9(9):457. doi: 10.3390/biom9090457

Figure 2.

Figure 2

Expression of Drosophila snoRNA:Me28S-U3344 and scaRNA:MeU2-C28 (wild-type and single-domain mutant variants) in transfected HeLa cells. (A) Diagram of the snoRNA expression construct used for transfection. (B) Northern blot analysis of MeU2-C28 expression; Me28S-U3344 served as an internal control. Note fully processed guide RNA molecules in all samples. The expression level of the functional single-domain MeU2-C28 guide RNA is similar to that of full-length wild-type. A non-functional, single-domain mutant (extreme mutant with additional mutations 9 and 10) accumulated to a much lower level, yet was still normally processed.