Table 1.

The SP/NK-1R system in human HB cell lines (HepT1; HepG2; HuH6).

NK-1R	Express mRNA for the NK-1R
	Expression of the TAC1R gene is increased
	Express full and truncated isoforms of the NK-1R. The HB cells express essentially the truncated form. Expression of the full form is higher in non-tumor cells
SP	A universal mitogen (at nanomolar concentration) of tumor cells, including HB cells
Non-peptide NK-1R antagonists	Antiproliferative action in a concentration-dependent manner: The higher the concentration, the greater the antitumor activity
	Induce cell death by apoptosis, cleavage of caspase-3and proteolysis of poly (ADP-ribose) polymerase
	Aprepitant (IC50) for HepT1 (31.1 µM), HuH6 (33.18 µM), HepG2 (38.61 µM)
	L-732,138 (IC50) for HepT1 (42 µM), HuH6 (41 µM), HepG2 (110 µM)
	L-733,060 (IC50) for HepT1 (15 µM), HuH6 (14 µM), HepG2 (17 µM)
	Co-administration of aprepitant and cytostatics exerts a synergistic antitumor effect
	Pretreatment of non-tumor cells (human embryonic kidney (HEK)-293) with aprepitant, protected these cells from cytostatic toxicity