Figure 4.

The lack of CX₃CR1 reduced the differentiation of monocyte-derived dendritic cells (moDCs) in response to liver inflammation. The liver distribution of CD11b⁺ myeloid dendritic cells, moDCs and monocytes were analyzed by flow cytometry in the livers of CX₃CR1^gfp/+ and CX₃CR1^gfp/gfp mice 36 h after receiving an acute dose of CCl₄. (A) The prevalence of CD11c⁺/MHCII^high/CD11b⁺ myeloid dendritic cells and CD11c⁺/MHCII^high/CD11b⁺/CD88⁺ moDCs in CX₃CR1^gfp/+ and CX₃CR1^gfp/gfp mice receiving CCl₄. (B) Hepatic distribution of Ly6G⁻/CD11b^high/Ly6C^high monocytes in control and CCl₄-treated CX₃CR1^gfp/+ and CX₃CR1^gfp/gfp mice. (C) Impaired expression of MHCII by CD11c⁺/CD11b⁺ myeloid cells in CCl₄-treated CX₃CR1^gfp/+ and CX₃CR1^gfp/gfp mice. The values are expressed as means ± SD of three different cell preparations or 5–6 animals.