Skip to main content
. 2019 Sep 19;8(9):1106. doi: 10.3390/cells8091106

Figure 1.

Figure 1

Inhibition of Wnt/β-catenin signaling increased 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2) expression in human intestinal cancer cells. (A,B) LS174T or Caco2 cells transfected with non-target control (NTC) siRNA or β-catenin (β-cat) siRNA were incubated for 48 h. (A) Western blot analysis was performed using the antibodies as indicated. HMGCS2 expression from three separate western blots was quantitated densitometrically and is expressed as fold change with respect to β-actin (n = 3, data represent mean ± SD; * p < 0.05 vs. NTC). (B) The level of HMGCS2 mRNA was assessed by real-time RT-PCR (n = 3, data represent mean ± SD; * p < 0.05 vs. NTC). (C,D) Inhibition of Wnt/β-catenin signaling increased the expression of HMGCS2 in LS174T and Caco2 cells. LS174T or Caco2 cells were treated with iCRT3 for 24 h (LS174T) or 48 h (Caco2). (C) Western blot analysis was performed using the antibodies as indicated. (D) HMGCS2 mRNA expression was assessed by real time RT-PCR (n = 3, data represent mean ± SD; * p < 0.05 vs. 0 μM iCRT3).