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. 2019 Sep 19;8(9):1106. doi: 10.3390/cells8091106

Figure 3.

Figure 3

Regulation of β-hydroxybutyrate (βHB) production by Wnt/β-catenin signaling in intestinal cells. (A) LS174T and Caco2 cells, transfected with NTC or β-catenin siRNA (β-cat) and incubated for 48 h or treated with iCRT3 for 24 h (LS174T) or 48 h (Caco2), were lysed and βHB content was measured using a βHB assay kit (n = 3, data represents mean ± SD; * p < 0.05 vs. NTC or 0 μM iCRT3). (B) LS174T and Caco2 cells were treated with 200 ng/mL Wnt3a or 40 mM LiCl (40 mM NaCl as control) for 24 h. Cell lysates were used for measurement of βHB content using a βHB assay kit (n = 3, data represents mean ± SD; * p < 0.05 vs. 0 ng/mL Wnt3a or 40 mM NaCl). (C) Mouse small intestinal organoids were treated with 100 ng/mL Wnt3a for 3 days. βHB content in cell lysates and conditioned media was determined using a βHB assay kit (n = 3, data represents mean ± SD; * p < 0.05 vs. 0 ng/mL Wnt3a).