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. 2019 Sep 19;8(9):1106. doi: 10.3390/cells8091106

Figure 5.

Figure 5

The expression and activity of PPARγ was regulated by the Wnt/β-catenin pathway in intestinal cancer cell lines. (A,B) LS174T or Caco2 cells were transfected with NTC or β-catenin siRNA and incubated for 48 h. (A) Western blot analysis was performed using the antibodies as indicated. PPARγ expression from three independent experiments was quantitated densitometrically and is expressed as fold change with respect to β-actin (n = 3, data represent mean ± SD; * p < 0.05 vs. NTC). (B) PPARγ mRNA was assessed by real-time RT-PCR (n = 3, data represent mean ± SD; * p < 0.05 vs. NTC). (C,D) LS174T or Caco2 cells were treated with NaCl (40 mM) as control or LiCl (40 mM) for 24 h. (C) Western blotting was performed using the antibodies as indicated. Densitometric analysis from three separate experiments was performed and PPARγ expression is represented as fold change with respect to β-actin (n = 3, data represent mean ± SD; * p < 0.05 vs. 40 mM/mL NaCl). (D) PPARγ mRNA was assessed by real-time RT-PCR (n = 3, data represents mean ± SD; * p < 0.05 vs. 40 mM/mL NaCl control). (E) LS174T cells were transfected with NTC or β-catenin siRNA for 48 h or treated with 40 mM NaCl or 40 mM LiCl for 24 h and PPARγ DNA-binding activity was determined as described under Materials and Methods (n = 3, data represents mean ± SD; * p < 0.05 vs. NTC or 40 mM NaCl). (F) Expression of FABP2 and PLIN2 mRNA was assessed by real-time RT-PCR. (n = 3, data represents mean ± SD; * p < 0.05 vs. NTC or 40 mM/mL NaCl).