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. 2019 Sep 18;11(9):1394. doi: 10.3390/cancers11091394

Figure 2.

Figure 2

Figure 2

Anti-cancer effect by transfection of siRNA against IL4Rα or IL13Rα1 in A498 and ACHN cells. Time-dependent anti-cancer effect by transfection of siRNA against IL4Rα or IL13Rα1 in A498 and ACHN cells for 24, 48, and 72 h incubation after transfection. Cell viability and proliferation rate was determined by WST-1 assay (A) and cell counting assay (B), respectively. This result is representative data from three biological replicates, and the error bar indicates standard error (STE). * indicates the p-value < 0.05. (C) Anti-colony formation ability by transfection of siRNA against IL4Rα or IL13Rα1 in A498 and ACHN cells was determined by colony formation assay for 14 days after transfection. These results are representative data from three biological replicates. Apoptosis in A498 and ACHN cells transfected with siRNA against IL4Rα or IL13Rα1 for 48 h after transfection was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (D) and Annexin V staining analysis (E). Cell cycle arrest was determined by cell cycle analysis (F). This result is representative data from three biological replicates. (G) Western blotting analysis of proteins related to apoptosis and cell cycle arrest in A498 and ACHN cells transfected with siRNA against IL4Rα or IL13Rα1 for 48 h after transfection. β-actin was used for a gel-loading control. Magnification for (D): ×20.