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. 2019 Sep 9;20(18):4442. doi: 10.3390/ijms20184442

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation of qSS1. (a) Graphic genotype of one line that carries an introduced NIP segment encompassing qSS1. Arrowhead (black) indicates centromeric region. (b) qSS1 is narrowed down to the interval flanked by the markers RM11698 and RM11716 using a derived F₂ population (n = 128). (c) Graphic genotype of NIL-qSS1⁹³¹¹. (d, e) Seed germination of NIL-qSS1⁹³¹¹ and NIL-qSS1^NIP after natural aging and artificial aging. NA and AA denote natural aging and artificial aging, respectively. Double asterisks in (e) indicate significant difference between NILs at p < 0.01.