Skip to main content
. 2019 Sep 4;20(18):4323. doi: 10.3390/ijms20184323

Figure 2.

Figure 2

Expression of TSPAN32 during human T cell activation. (A) Expression of TSPAN32 was evaluated via real-time PCR, in CD4+ T effector cells stimulated at different time points with anti-CD3/CD28 antibodies or (B) with anti-CD3 alone or in combination with anti-CD28, anti-CTLA4, anti-PD1 and anti-ICOS antibodies for 36 h (n = 3 independent replicates). (C) TSPAN32 levels in CD4+ effector T cells upon anti-CD3/CD28 stimulation, in the presence or absence of rapamycin 200 nM, were evaluated by real-time PCR (n = 3 independent replicates). (D) TSPAN32 expression levels were evaluated at different time points upon activation of Treg cells via real-time PCR (n = 3 independent replicates). (E) TSPAN32 protein levels were determined by western blot upon activation of effector and regulatory T cells at different time points (pooled proteins of cells from 3 healthy donors). Data are shown as normalized mean ± SD and statistical analysis performed using one-way ANOVA followed by Bonferroni multiple test correction.