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Effect of TSPAN32 overexpression in Jurkat cells. Following transient transfection of Jurkat cells with a DNA plasmid encoding for TSPAN32 (pTSPAN32) or the empty plasmid, cells were stimulated with anti-CD3/CD28 for 24 h and the concentrations of TNF-alpha and IFN-gamma in the supernatant were determined by ELISA. Data are shown as normalized mean ± SD and statistical analysis performed using one-way ANOVA followed by Bonferroni multiple test correction.
