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. 2019 Sep 18;20(18):4634. doi: 10.3390/ijms20184634

Figure 3.

Figure 3

FGF23 induces RAAS-associated genes in neonatal rat ventricular myocytes (NRVM) and cardiac fibroblasts (NRCF) in vitro as shown by quantitative real-time PCR. (A–D) In NRVM, FGF23 treatment increases the mRNA expression of angiotensinogen (Agt) and renin (Ren), whereas the effects of FGF23 on angiotensin converting enzyme (Ace) and angiotensin II receptor type 1 (AT1R) expression levels are not statistically significant. (E) FGF23 and aldosterone (Aldo) induce the mRNA expression of neutrophil gelatinase-associated lipocalin (Ngal) in NRVM. (F–I) In NRCF, Agt, Ace and Ren mRNA expressions are enhanced after FGF23 treatment, but FGF23 does not significantly induce AT1R. (J) Ngal mRNA expression is elevated upon FGF23 and aldosterone stimulation. All values are shown as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus control; n = 6–8 independent cell isolations.