Skip to main content
. 2019 Sep 8;11(9):1330. doi: 10.3390/cancers11091330

Figure 1.

Figure 1

Functional overexpression of multidrug resistance protein 2 (MRP2) in human colorectal cancer Caco-2 cells and pancreatic cancer PANC-1 cells. (A,B) show high ABCC2 mRNA expression in Caco-2 and PANC-1 cells (both in red color) compared to other colorectal and pancreatic cancer cell lines from Wagner dataset stored in ONCOMINE (https://www.oncomine.org). (C,D) show MRP2 protein detected in representative flow cytometry histogram of cell surface staining using the anti-MRP2 primary antibody (red) and isotype control IgG2a (green) on Caco-2 and PANC-1 cells. Both the primary antibody and isotype control were labelled with Alexa Fluor 488 secondary antibody. The x-axis is the fluorescence signal intensity displayed in a liner log scale. Functional expression of MRP2 detected by CDCF accumulation in Caco-2 cells (E) and PANC-1 cells (F) at different time points in the presence and absence of 60 μM myricetin. All data are normalized to the fluorescence intensity determined at 5 min in the absence of myricetin. The bar represents the mean and standard errors of the mean values from at least three independent experiments. Asterisks are p values (*, p < 0.05; ***, p < 0.001) for differences at each time point from Sidak post-tests that followed a two-way analysis of variance (ANOVA).