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. 2019 Sep 10;20(18):4469. doi: 10.3390/ijms20184469

Figure 7.

Figure 7

Analysis of NO epifluorescence signal, due to diaminofluorescein-FM diacetate (DAF-FMDA) treatment, in the basal hypocotyl of Col-0 (wt) seedlings dark-grown for 22 DAS. (A) Absence of epifluorescence signal in the pericycle periclinal derivatives occasionally formed in hormone-free (HF) condition. (B) Absence of epifluorescence signal in an adventitious root primordium derived from anticlinal pericycle divisions in HF condition. (C,D) Weak signal in correspondence of cells derived from periclinal (C) or anticlinal (D) divisions of the pericycle in seedlings treated with 50 µM sodium nitroprusside (SNP). (E,F) Strong NO signal in the pericycle cells (E), and their first periclinal derivatives (F; arrow in the inset) in 50 µM SNP + 10 µM JAMe (SNP + 10JAMe)–treated seedlings. (G) Absence of epifluorescence signal in the pericycle after 100 µM 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) application to 10 µM JAMe-treated seedlings (cPTIO + 10JAMe). (H) Strong NO signal in pericycle periclinal derivatives in the hypocotyl of a seedling treated with SNP + 10JAMe. The signal disappears in the differentiated xylary elements (the arrow indicates an ectopic protoxylem element). (I) Strong NO signal in pericycle anticlinal derivatives occasionally formed after SNP + 10JAMe application. (J) Weak NO signal in pericycle-derived cells in 50 µM SNP + 0.1 µM ACC (SNP + ACC)-treated seedlings. (K) Absence of NO signal in the hypocotyl of seedling cultured with 100 µM cPTIO + 0.1 µM ACC (cPTIO + ACC). (L,M) Epifluorescence signal in the hypocotyl of seedlings treated with 10 µM IBA (IBA; L) or 10 µM IBA + 10 µM JAMe (IBA + 10 JAMe; M). The signal intensity increases in the presence of JAMe. All the insets show bright field images of whole-mount seedling hypocotyls. Scale bars = 10 µm (A,B,D–I,K–M, Insets in A–F,I,K,L), 20 µm (C,J, Insets in G,H,M), 30 µm (Inset in J).