Table 1.
Quantification of ectopic xylary elements (XEs) detected in the basal hypocotyl of Col-0 and ein3eil1 seedlings. Mean number (± standard error (SE)) of XEs and percentage of ectopic protoxylem and metaxylem elements detected in an area of 150 × 150 µm2 in the middle portion of the basal hypocotyl with different treatments. ACC, IBA and IAA were always used at 0.1 µM, 10 µM and 10 µM, respectively. Different letters among values within the same column indicate significant differences at least at p < 0.05. The same letter indicates no statistical difference. One-way ANOVA followed by Tukey’s post-test. n = 30.
| Treatment | Col-0 Ectopic XEs Mean Number (± SE) |
ein3eil1 Ectopic XEs Mean Number (± SE) |
Col-0 Ectopic Protoxylem % |
Col-0 Ectopic Metaxylem % |
ein3eil1 Ectopic Protoxylem % | ein3eil1 Ectopic Metaxylem % |
|---|---|---|---|---|---|---|
| HF (control) | 0.5 ± 0.2 a | 0.6 ± 0.3 a | 100 | 0 | 100 | 0 |
| 0.01 µM JAMe | 1.3 ± 0.3 a | 2.3 ± 0.7 b | 100 | 0 | 100 | 0 |
| 1 µM JAMe | 1.0 ± 0.3 a | 2.4 ± 0.7 b | 100 | 0 | 100 | 0 |
| 10 µM JAMe | 2.8 ± 0.3 b | 2.5 ± 0.4 b | 100 | 0 | 100 | 0 |
| 0.1 µM ACC | 1.1 ± 0.3 a | 0.6 ± 0.2 a | 45.5 | 54.5 | 100 | 0 |
| 10 µM JAMe + ACC | 0.8 ± 0.2 a | 2.4 ± 0.3 b | 100 | 0 | 100 | 0 |
| 10 µM IBA | 4.4 ± 0.6 b | 2.0 ± 0.1 b | 0 | 100 | 100 | 0 |
| IBA + 0.01 µM JAMe | 3.5 ± 0.4 b | 2.4 ± 0.3 b | 17.6 | 82.4 | 20.7 | 79.3 |
| IBA + 1 µM JAMe | 3.7 ± 0.8 b | 2.3 ± 0.4 b | 22.7 | 77.3 | 22.2 | 77.8 |
| IBA + 10 µM JAMe | 4.0 ± 0.6 b | 3.6 ± 0.2 b | 61.9 | 38.1 | 50 | 50 |
| 10 µM IAA | 2.8 ± 0.5 b | 2.0 ± 0.4 b | 100 | 0 | 100 | 0 |
| IAA + 0.01 µM JAMe | 2.5 ± 0.4 b | 1.8 ± 0.4 b | 100 | 0 | 35 | 65 |
| IAA + 1 µM JAMe | 2.5 ± 0.2 b | 2.2 ± 0.3 b | 33.3 | 66.7 | 25 | 75 |
| IAA + 10 µM JAMe | 2.6 ± 0.5 b | 3.8 ± 0.8 b | 7.7 | 92.3 | 9 | 91 |