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. 2019 Sep 17;20(18):4603. doi: 10.3390/ijms20184603

Figure 5.

Figure 5

The deletion of the −72.8 kb region reduced the Tpsb2 and Tpsg1 gene expression in BRC6 mast cells. (a,b) Results of qRT-PCR of the Tpsb2 and Tpsg1 gene transcript in BRC6 cells transfected with either control, GATA1 or GATA2 siRNA (a) or pEF plasmid DNAs (b). In the plasmid transfection, pmaxGFP was co-transfected with pEF plasmid DNAs, and green fluorescent protein (GFP)-positive cells were sorted at 24 h after transfection. (c) The results of qRT-PCR of mRNAs transcribed from the indicated genes in genome-edited BRC6 cells. The samples were prepared from undeleted (WT) and homozygous (Δ−72.8 kb) clones. n = 6 for each group. *, p < 0.05; **, p < 0.01; n.s., not significant. n = 5.