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. 2019 Sep 17;20(18):4603. doi: 10.3390/ijms20184603

Table A3.

Sequences of oligonucleotide used for CRISPR Cas9 genome editing.

Applied Oligonucleotide Sequences of Oligonucleotide (5′ to 3′)
sgRNAs 5′-fwd CACCGTCTTACTGTAGATTTAAGC
5′-rev AAACGCTTAAATCTACAGTAAGAC
3′-fwd CACCGACATTAGAACACTATTAGTA
3′-rev AAACTACTAATAGTGTTCTAATGTC
5′ arm Fwd TAATGGGCCCACAGGGCCTCATTTTGTAGG
Rev ACCGATCGATGCTTAAATCTACAGTAAGAC
3′ arm Fwd TATGGGATCCACTACTTTTACACTGCCTCA
Rev ACTTGCGGCCGCTACATGTTGGCACAAGCCTG