Table A3.
Applied Oligonucleotide | Sequences of Oligonucleotide (5′ to 3′) | |
---|---|---|
sgRNAs | 5′-fwd | CACCGTCTTACTGTAGATTTAAGC |
5′-rev | AAACGCTTAAATCTACAGTAAGAC | |
3′-fwd | CACCGACATTAGAACACTATTAGTA | |
3′-rev | AAACTACTAATAGTGTTCTAATGTC | |
5′ arm | Fwd | TAATGGGCCCACAGGGCCTCATTTTGTAGG |
Rev | ACCGATCGATGCTTAAATCTACAGTAAGAC | |
3′ arm | Fwd | TATGGGATCCACTACTTTTACACTGCCTCA |
Rev | ACTTGCGGCCGCTACATGTTGGCACAAGCCTG |