Figure 5.

Defense-related traits identified in BEC1019-overexpressing and -silenced plants. (A) The results of qRT-PCR analyses showing the transcript levels of wheat defense marker genes TaPR2 and TaPR10 in BEC1019-overexpressing and RNAi plants. Double asterisks indicate significant differences relative to the control plants (p ≤ 0.01, according to t tests). (B) The leaves of wild-type wheat and BEC1019-overexpressing and RNAi plants were challenged with Blumeria graminis f. sp. tritici (Bgt) and sampled at 3 days post-infection (dpi) with DAB staining. (C) The leaves of wild-type wheat and BEC1019 transgenic plants were challenged with Bgt, sampled at 1 dpi, and used to observe cell death, which was detected by staining with trypan blue.