. 2019 Sep 6;20(18):4376. doi: 10.3390/ijms20184376

Table 1.

Primers names and sequences used in this study.

Primer Name	Primer Sequence (5′-3′)	Primers Purpose
BEC1019-F	ATGCAGTCTGTATTGCTTTT	Homolog gene cloning of BEC1019
BEC1019-R	CTAGACACAATGAACCTCGC	Homolog gene cloning of BEC1019
BEC1019-F1	GGGGACAGTTTGTACAAAAAAGCAGGCTTCATGCAGTCTGTATTGCTTTT	Construction of CTAPi-GW-3HA-BEC1019 vector
BEC1019-R1	GGGGACCACTTTGTACAAGAAAGCTGGGTGACACAATGAACCTCGCCAT	Construction of CTAPi-GW-3HA-BEC1019 vector
BEC1019-F2	GGGGACAGTTTGTACAAAAAAGCAGGCTTCGTGATGACCCGGACAAAA	Construction of pCAMBIA2301-BEC1019-RNAi vector
BEC1019-R2	GGGGACCACTTTGTACAAGAAAGCTGGGTAGGGCATCTTGGTAACCA	Construction of pCAMBIA2301-BEC1019-RNAi vector
BEC1019-F3	GGGGACAGTTTGTACAAAAAAGCAGGCTTCGTGATGACCCGGACAAAA	Construction of CTAPi-GW-3HA-BEC1019-RNAi vector
BEC1019-R3	GGGGACCACTTTGTACAAGAAAGCTGGGTAGGGCATCTTGGTAACCA	Construction of CTAPi-GW-3HA-BEC1019-RNAi vector
BEC1019-F4	TCATGTGGACATCGTCGGTC	q RT-PCR analysis of BEC1019 over-expression wheat lines
BEC1019-R4	CACGCTGATGTCAAACGCAT	q RT-PCR analysis of BEC1019 over-expression wheat lines
BEC1019-F5	AATGTGCAACCGAGAACCGA	q RT-PCR analysis of BEC1019 silencing wheat lines
BEC1019-R5	TCCTCCAAAGGAAGCCGTTC	q RT-PCR analysis of BEC1019 silencing wheat lines
TaPR2-F	CCGGCCATACTACCCGGC	q RT-PCR analysis for TaPR2
TaPR2-R	ACACCTTGATGGCGCTGAGA	q RT-PCR analysis for TaPR2
TaPR10-F	ACGGAGCGGATGTGGAAG	q RT-PCR analysis for TaPR10
TaPR10-R	GCCACCTGCGACTTGAGC	q RT-PCR analysis for TaPR10
TaActin-F	CCAGGTATCGCTGACCGTAT	Reference gene of wheat
TaActin-R	GCTGAGTGAGGCTAGGATGG	Reference gene of wheat
Bgt-EF1a-F	GTCGGATTTAACCCCAAGGT	Reference gene of Bgt
Bgt-EF1a-R	TTTATCGGTAGGGCGACTTG	Reference gene of Bgt
Ggt-18S rRNA-F	CGAACTCGGTCGTTTAGAGG	Reference gene of Ggt
Ggt-18S rRNA-R	GGTATGTTCACAGGGGTTGG	Reference gene of Ggt