Figure 4.

Mitochondrial structural defects in slo¹ mutants. (A) Wt mitochondria from indirect flight muscles at age 1 day show normal cristae organization. The slo¹ mutant mitochondria also show normal structure but there are increased numbers of vacuoles in the muscles (D). (B) Wt mitochondria at the age of day 30 also show a normal cristae organization. However, the slo¹ mutant mitochondria show disorganized cristae and swollen mitochondria (E). (C) Mitochondria of very old flies (at day 60) in wt show swirling of cristae, a phenotype characteristic of old age but occasionally young (day 1) slo¹ flies also show such swirls (F), indicated by white arrows. (G) The Average area indicated by histogram showed no difference in day 1 mitochondria in between wt (black) and slo¹ (gray) but significant (* p < 0.05) difference at day 30.