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. 2019 Sep 12;20(18):4521. doi: 10.3390/ijms20184521

Figure 3.

Figure 3

Mapping of core cis elements in the CYP6CY3 promoter region by 5′ progressive deletions (A), internal deletions (B) and mutations (C,D), and the effect of (AC)n repeat length on promoter activity (E) and its nicotine-inducible activity (F).The name of each CYP6CY3 promoter-pGL3 construct contains a “P” and a pair of parentheses with two numerals separated by a dash to specify the 5′ and 3′ positions of the corresponding promoter fragment. For internal deletion or substitution mutation constructs, the name of the deleted or mutated sequence and a “-del” or “-mut” are added after the parentheses. The deleted region was ~-221–-138 as shown in Figure S2. The mutated nucleotides in the (AC)n repeats are bold and shown in italics. The final concentration of nicotine for induction was 24.7 μM/L. Different letters on the bars of the histogram indicate significant differences based on an ANOVA, followed by Tukey’s HSD multiple comparison test (p < 0.05).