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. 2019 Sep 12;20(18):4521. doi: 10.3390/ijms20184521

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of AhR and ARNT modulation on CYP6CY4 expression in M. persicae nicotianae. (A,B) The relative CYP6CY3 and CYP6CY4 expression levels in M. persicae nicotianae orally treated with dsRNA (100 ng/μL dsRNA-CYP6CY4 or dsRNA-CYP6CY3) after 48 h [23]. (C) Orally mediated dsRNA knockdown of AhR or ARNT (100 ng of AhR or ARNT dsRNA) impacts CYP6CY4 transcriptional level. The housekeeping genes actin and para were used as internal reference genes [24]. Different letters on the bars of the histogram indicate significant differences based on ANOVA followed by Tukey’s HSD multiple comparison test (p < 0.05). ** Significant difference by Student’s t-test (p < 0.01).