Figure 1.

Viral metagenomics ring trial design. The figure shows the complete workflow from the sample to the report, which may include enrichment of viral particles, followed by nucleic acids (NA) extraction and viral nucleic acid enrichment, library preparation, sequencing, classification of reads, summary statistics per virus and final interpretation. For increment 1, five samples consisting of biological material were sent to participants (left orange arrow). For increment 2, participants received 13 FASTQ datasets (right orange arrow). Output files are listed at the top after each workflow step (blue arrow).