Figure 7.

HN inhibits AgNPs-induced ROS generation, lipid peroxidation, nitric oxide and protein carbonyl content in SH-SY5Y cells. SH-SY5Y cells were pretreated with or without 10 μg/mL HN for 24 h. Cells were then treated with 10 μg/mL HN and/or 10 μg/mL AgNPs for 24 h. (A). ROS generation was measured in micromoles per min. (B). The concentration of MDA was measured using a thiobarbituric acid-reactive substances assay and expressed as nanomoles per milliliter. (C) NO production was quantified spectrophotometrically using the Griess reagent and expressed as micromoles (D). Protein carbonyl content was measured and expressed as nanomoles. The results are expressed as the mean ± standard deviation of three independent experiments. The treated groups showed statistically significant differences from the control group based on Student’s t-test (* p < 0.05).