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. 2019 Sep 9;20(18):4439. doi: 10.3390/ijms20184439

Figure 9.

Figure 9

HN protects SH-SY5Y cells from AgNPs-induced mitochondrial dysfunctions. SH-SY5Y cells were pretreated with or without 10 μg/mL HN for 24 h. Cells were then treated with 10 μg/mL HN and/or 10 μg/mL AgNPs for 24 h. (A) Mitochondrial membrane potential (MMP) was determined using the cationic fluorescent indicator JC-1 (B). Intracellular ATP content was determined according to the manufacturer’s instructions (Sigma-Aldrich, St. Louis, MO, USA; Catalog Number MAK135) (C) Mitochondrial DNA copy number was determined by quantitative real-time PCR and was expressed relative to control cells (D) Expression of mRNA levels of PGC-1alpha was determined by the quantitative real-time PCR analysis. Results are expressed as mean ± standard deviation of three independent experiments. There was a significant difference in treated cells compared to untreated cells based on Student’s t-test (* p < 0.05).