Figure 10.

Effect of HN on AgNPs mediated modulation of the expression of apoptotic, antiapoptotic and ER stress sensor genes in SH-SY5Y cells. SH-SY5Y cells were pretreated with or without 10 μg/mL HN for 24 h. Cells were then treated with 10 μg/mL HN and/or 10 μg/mL AgNPs for 24 h. Relative messenger RNA (mRNA) expression of apoptotic, antiapoptotic and ER stress sensor genes was analyzed by quantitative reverse-transcription polymerase chain reaction. After 24 h treatment expression fold level was determined as fold changes in reference to expression values against GAPDH. Results are expressed as fold changes. Results are expressed as the mean ± standard deviation of three independent experiments. There was a significant difference in treated cells compared to untreated cells based on Student’s t-test (* p< 0.05).