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After extraction, microextraction probes were placed in acetonitrile-water (75:25 by volume) for 10, 20, 30 and 45 min to determine the optimal desorption time. As desorption time increased, % carryover decreased (experiments were performed in triplicate).
AEA: Anandamide; 2-AG: 2-arachidonoyl glycerol.
