Figure 4. . Extraction time profile for anandamide and 2-arachidonoyl glycerol.

The experiments were performed in vitro using solutions of 10 ng/ml of each compound in 1% agarose gel; groups of three probes were collected and analyzed at the following time points: 5, 10, 15, 20, 30, 45 and 60 min. ‘n’ represents the amount extracted and ‘n0’ the maximum amount extracted (at equilibrium); error bars represent one standard deviation (experiments were performed in triplicate).

2-AG: 2-arachidonoyl glycerol; AEA: Anandamide.