Table 2.
Identification of IgE-binding proteins from Sinapis alba and Brassicae juncea mustard by mass spectrometry analysis.
| Band Number a | MW on SDS-PAGE (KDa) | Protein Name b | Protein Accession Numbers | Protein MW (KDa) | Exclusive Unique Peptide Count | Percentage Sequence Coverage c |
|---|---|---|---|---|---|---|
| Sinapis alba (AC Pennant) | ||||||
| S1 | 16 | ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.4 | 5 | 23.40% |
| S2 | 28 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 16 | 39.40% |
| S3 | 30 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 16 | 39.40% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 13 | 30.10% | ||
| S4 | 44 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 19 | 44.30% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 14 | 29.70% | ||
| S5 | 75 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 18 | 44.30% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 7 | 23.40% | ||
| S6 | 107 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 21 | 46.50% |
| S7 | 223 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 20 | 39.40% |
| BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 56.5 | 20 | 34.40% | ||
| RS1 | 9.5 | ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.4 | 7 | 26.90% |
| RS2 | 12 | ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.4 | 9 | 57.90% |
| RS3 | 20 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 19 | 36.10% |
| BRANA Cruciferin (Fragment) [Brassica napus (Rape)] | Q7XB53 | 51.3 | 8 | 23.20% | ||
| RS4 | 28 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 14 | 25.90% |
| CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 7 | 27.50% | ||
| RS5 | 34 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 14 | 25.90% |
| SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLV9 | 57.9 | 5 | 34.60% | ||
| RS6 | 44 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 18 | 46.70% |
| RS7 | 55 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 15 | 37.60% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 10 | 25.20% | ||
| RS8 | 62 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 20 | 45.10% |
| RS9 | 75 | Q2TLW0_SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 16 | 40.20% |
| BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 58.5 | 12 | 20.80% | ||
| RS10 | 82 | Q2TLW0_SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 14 | 37.10% |
| RS11 | 118 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 12 | 27.50% |
| RS12 | 140 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 9 | 24.50% |
| Brassicae juncea (AC Vulcan) | ||||||
| B1 | 15.5 | Allergen Bra j 1-E [Brassica juncea (Indian mustard)] | P80207 | 14.6 | 5 | 51.90% |
| OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 19.9 | 15 | 46.80% | ||
| B2 | 17 | BRANA Oleosin S3-1 [Brassica napus (Rape)] | C3S7F8 | 19.6 | 10 | 41.70% |
| OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 19.9 | 16 | 46.80% | ||
| B3 | 26 | BRANA Caleosin CLO1-2 [Brassica napus (Rape)] | C3S7H5 | 28.1 | 7 | 35.90% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 21 | 49.50% | ||
| B4 | 30 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 12 | 47.20% |
| OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 19.9 | 11 | 46.30% | ||
| B5 | 29 | BRANA Cruciferin (Fragment) [Brassica napus (Rape)] | Q7XB53 | 51.3 | 4 | 8.80% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 8 | 24.30% | ||
| B6 | 34 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 7 | 20.80% |
| CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 3 | 21.80% | ||
| B7 | 42.5 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 12 | 45.20% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 20 | 52.30% | ||
| B8 | 68 | CRU3_BRANA Cruciferin CRU1 [Brassica napus] | P33525 | 56.5 | 14 | 52.80% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus] | P33522 | 51.4 | 13 | 40.90% | ||
| B9 | 200 | Malate synthase. glyoxysomal [Brassica napus (Rape)] | P13244 | 63.7 | 14 | 23.00% |
| BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 58.5 | 23 | 44.20% | ||
| RB1 | 9.5 | Allergen Bra j 1-E [Brassica juncea (Indian mustard)] | P80207 | 14.6 | 2 | 38.00% |
| RB2 | 12 | Allergen Bra j 1-E [Brassica juncea (Indian mustard)] | P80207 | 14.6 | 5 | 68.20% |
| ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.5 | 4 | 35.20% | ||
| RB3 | 22 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 11 | 39.50% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 11 | 35.30% | ||
| RB4 | 27 | BRANA Caleosin CLO1-2 [Brassica napus (Rape)] | C3S7H5 | 28.1 | 8 | 37.60% |
| OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 20.0 | 6 | 40.40% | ||
| BRAJU Glutathione S-transferase 3 [Brassica juncea] | Q7XZT2 | 24.1 | 7 | 46.00% | ||
| RB5 | 30 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 17 | 42.60% |
| RB6 | 30 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 12 | 45.20% |
| CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 15 | 38.70% | ||
| RB7 | 34 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 8 | 28.90% |
| BRAJU Cysteine synthase [Brassica juncea] | O23733 | 33.9 | 8 | 33.20% | ||
| RB8 | 47 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 25 | 54.00% |
| BRANA Cruciferin (Fragment) [Brassica napus (Rape)] | Q7XB53 | 51.3 | 14 | 39.70% | ||
| RB9 | 52 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 16 | 45.40% |
| BRACM Enolase [Brassica campestris (Field mustard)] | Q6W7E8 | 47.4 | 21 | 61.00% | ||
| RB10 | 62 | BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 58.5 | 13 | 30.00% |
| RB11 | 76 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 8 | 30.10% |
| SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 5 | 15.30% | ||
a Band numbers correspond to the IgE-binding bands detected in Figure 5; b only protein identifications with 100% probability were retained; c total percentage of proteins amino acid sequence covered by the identified peptides in MS/MS analysis.