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. 2019 Aug 23;11(9):1233. doi: 10.3390/cancers11091233

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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T-STAR interacts with metadherin and is overexpressed in prostate cancer. (A) Expression of YTHDC1, Sam68, T-STAR and metadherin were examined in protein lysates from prostate cancer cell lines PNT1a, LNCaP, C4-2, C4-2B and PC3. (B) Endogenous metadherin was immunoprecipitated from 1mg LNCaP protein lysate with 10µg protein lysate used as input and rabbit IgG as a negative control. (C) Endogenous SAM68 was immunoprecipitated from 1 mg LNCaP protein lysate. 10 µg protein lysate was used as input, rabbit IgG as a negative control. White arrow indicates the band corresponding to metadherin.