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. 2019 Sep 11;20(18):4487. doi: 10.3390/ijms20184487
ACI autologous chondrocyte implantation
3D three-dimensional
ECM extracellular matrix
MSC mesenchymal stem cells
BMSC bone-derived mesenchymal stem cells
hADSC human adipose-derived stem cells
h hour
min minutes
CHI chitosan
GAG glycosaminoglycan
HA hyaluronic acid
DMEM Dulbecco’s modified eagle medium
SEM scanning electron microscope
qRT-PCR quantitative real-time polymerase chain reaction
hTGF-β3 human transforming growth factor beta 3
hBMP-6 human bone morphogenetic protein 6
WST-1 water soluble tetrazolium-1
NCHI chitosan in normal growth medium
NCHI/HA chitosan/hyaluronic acid in normal medium
CCHI chitosan in chondrogenic growth medium
CCHI/HA chitosan/hyaluronic acid in chondrogenic medium
NP pellet in normal growth medium
CP pellet in chondrogenic growth medium
ACAN aggrecan
COL1A1 collagen type I
COL2A1 collagen type II
COL10A1 collagen type X
SOX9 SRY-box 9
COMP cartilage oligomeric matrix protein
NRQ normalized relative quantity
TBP TATA sequence binding protein
SDHA succinate dehydrogenase complex flavoprotein subunit A
RPLP0 ribosomal protein lateral stalk subunit P0
RPL13a ribosomal protein L13a
ROCK Rho/Rho associated kinase
PI3K phosphatidylinositol-3-kinase
RHAMM receptor for hyaluronic acid mediated mobility
PBS phosphate buffered saline
FCS fetal calf/bovine serum
SD standard deviation