Table 1.
Gene specific primers used for quantitative real-time PCR.
| Gene | Forward Primer (5′–3′) | Reverse Primer (3′–5′) | Accession Nr. | Amplicon Size (bp) |
|---|---|---|---|---|
| COL2A1 | GCCCAGTTGGGAGTAAGT | CACCAGGATTGCCTTGAA | NM_001844.4 | 106 |
| COL1A1 | GCTGGTCCTCCAGGTGAA | GGGGACCAACAGGACCA | NM_000088.3 | 159 |
| COL10A1 | TGGCCTGCCTGACTTTA | AATGTCCAGCTCACTGGA | NM_000493.3 | 151 |
| ACAN | ACCCAAGGACTGGAATCT | CCTGATCCAGGTAGCCTT | NM_001135.3 | 149 |
| COMP | TGCACCGACGTCAACGA | CCGGGTGTTGATGCACA | NM_000095.2 | 231 |
| SOX9 | GTGGCTGTAGTAGGAGCT | GCGAACGCACATCAAGA | NM_000346.3 | 155 |
| ACTB | CTGCCCTGAGGCACTC | GTGCCAGGGCAGTGAT | NM_001101.3 | 197 |
| RPLP0 | CAACCCAGCTCTGGAGA | CAGCTGGCACCTTATTGG | BC001834.2 | 116 |
| TBP | CACTTCGTGCCCGAAAC | GCCAGTCTGGACTGTTCT | BC110341.1 | 121 |
| POLR2e | CTATCTGGTGACCCAGGA | CTGCAGAAACTGCTCCA | J04965.1 | 322 |