Figure 5.

Necrosis and apoptosis induced by ZVI@mSiO₂ in OEC-M1 cells could be rescued by the lysosome inhibitor NH₄Cl, iron chelator DFO, antioxidant vitamin C, and the necrosis inhibitor necrostatin-1. (a) The flow cytometry data showed that cotreatment with ZVI@mSiO₂ and NH₄Cl or vitamin C could reduce the percentage of the necrotic and the apoptotic population after 24-h treatment, and a combination of both compounds drastically decreased the necrotic and apoptotic populations. (b) The cytotoxicity of ZVI@mSiO₂ was also significantly compromised by cotreatment with DFO, NH₄Cl or vitamin C or both after 24-h treatment. (c) The cytotoxic effect caused by ZVI@mSiO₂ treatment was successfully rescued via necrostatin cotreatment in a dose dependent manner.