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. 2019 Sep 4;11(9):1303. doi: 10.3390/cancers11091303

Figure 2.

Figure 2

Cell cycle changes of manoalide-treated oral cancer (Ca9-22 and CAL 27) cells. (A) Statistical results of the subG1 (%) for manoalide-treated oral cancer cells in Figure S2A. Cells were treated with 0, 5, 10, and 20 μM of manoalide for 24 h. (B) Statistical result of the subG1 (%) for NAC, Z-VAD, and/or manoalide-treated oral cancer cells in Figure S2B. Cells were pretreated with 8 mM, 1 h for NAC or 100 μM and 2 h for Z-VAD, and they were then post-incubated with 10 μM of manoalide for 24 h. Data, means ± SDs (n = 3). Data were analyzed by one-way ANOVA with Tukey HSD Post Hoc Test. Data showing no overlapping same small letters represent significant difference (p < 0.05–0.001).