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. 2019 Sep 4;11(9):1303. doi: 10.3390/cancers11091303

Figure 4.

Figure 4

ROS changes in manoalide-treated oral cancer (Ca9-22 and CAL 27) and normal oral (HGF-1) cells. Cells were treated with different concentrations of manoalide for 10 min. (A). Statistical results of ROS (+) (%) for manoalide-treated oral cancer and oral normal cells in Figure S7A. (B) Statistical results in NAC, Z-VAD, and/or manoalide-treated oral cells in Figure S7B. Cells were pretreated with NAC (8 mM, 1 h) or Z-VAD (100 μM, 2 h), and posttreated with manoalide (10 μM, 10 min). Data were analyzed by one-way ANOVA with Tukey HSD Post Hoc Test. Data, means ± SDs (n = 3). Data showing no overlapping same small letters represent significant differences (p < 0.05–0.001).