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. 2019 Sep 4;11(9):1303. doi: 10.3390/cancers11091303

Figure 5.

Figure 5

Change of mitochondrial superoxide (MitoSOX) production in manoalide-treated oral cancer (Ca9-22 and CAL 27) cells. (A) Statistical results of MitoSOX (+) (%) for manoalide-treated oral cancer cells in Figure S8A. Cells were treated with different concentrations of manoalide for 24 h. (B) Statistical results of positive control of MitoSOX (+) (%) for oral cancer cells in Figure S8B. Cells were treated with betulinic acid (BA; 25 μM, 24 h) as the positive control treatment for comparison to manoalide (10 μM, 24 h). (C) Statistical results of MitoSOX (+) (%) in MitoSOX inhibitor (MitoTEMPO) and/or manoalide-treated oral cells in Figure S8C. Cells were pretreated with MitoTEMPO (20 μM, 1 h) and posttreated with manoalide (10 μM, 24 h). Data were analyzed by one-way ANOVA with Tukey HSD Post Hoc Test. Data, means ± SDs (n = 3). Data showing no overlapping same small letters represent significant difference (p < 0.05–0.001).