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. 2019 Sep 4;11(9):1303. doi: 10.3390/cancers11091303

Figure 7.

Figure 7

Change of γH2AX DNA damage in manoalide-treated oral cancer (Ca9-22 and CAL 27) cells. Cells were treated with the indicated concentrations of manoalide for 24 h. (A) Statistical results of γH2AX (+) (%) for manoalide-treated oral cancer cells in Figure S10A. (B) Statistical results of γH2AX (+) (%) in NAC, Z-VAD, and/or manoalide-treated oral cancer cells in Figure S10B. Cells were pretreated with 8 mM, 1 h for NAC or 100 μM, 2 h for Z-VAD, and then post-incubated with 10 μM of manoalide for 24 h. (C) Statistical results of γH2AX (+) (%) in MitoSOX inhibitor (MitoTEMPO) and/or manoalide-treated oral cancer cells in Figure S10C. Cells were pretreated with MitoTEMPO (20 μM, 1 h) and posttreated with manoalide (10 μM, 24 h). Data were analyzed by one-way ANOVA with Tukey HSD Post Hoc Test. Data, means ± SDs (n = 3). Data showing no overlapping same small letters represent significant difference (p < 0.05–0.001).