Figure 6.

Expression of glucose and lactate transporters and glycolytic enzymes in MCF7^IGF1R-ve/IR-A cells. (A) Cells were cultured in 10% charcoal stripped-FBS for 48 h, exposed to 10 nM insulin or IGF2 for 20 h or left untreated (NT), and then processed for mRNA expression for glucose and lactate transporters and glycolytic enzymes by qRT-PCR, as indicated. GAPDH was used as the housekeeping control gene. Values are means ± SEM of three separate experiments. (B) MCF7^IGF1R-ve/IR-A cells, cultured as in (A) and exposed to either insulin or IGF2 or untreated (NT) were lysed, analyzed by SDS-PAGE and immunoblotted with the indicated primary antibodies. Phosphorylation of IGF1R/IR, AKT and ERK1/2 was evaluated to assess signal activation. β-actin was used as control for protein loading. Blots are representative of three independent experiments. (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001).