Figure 1.

Blockade of triggering receptor expressed on myeloid cells (TREM)2 promotes the growth of HT29 colon cancer cells. (A) Using RT-PCR, mRNA expression levels of DNAX adaptor protein-12 (DAP12) and TREM2 were measured in MC38, HCT116, and HT29 colon cancer cell lines, and the Raw264.7 macrophage cell line was used as a positive control. (B) HT29 cells (1 × 10⁶ cells) were plated in each well of six-well plates and treated with H-Ig or T-Ig (2 μg/mL) for 48 h. Cell number was assessed by the trypan blue dye exclusion method, (C) and cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium) (MTS) assay. (D) Cell cycle analysis in HT29 cells treated with hIg or TREM2-Ig (2 μg/mL) was performed by flow cytometry. (E) Cell cycle-related protein expression in HT29 cells (F) and in cells isolated from the colons of WT littermate and TREM2-TG mice was analyzed by Western blot. The data are representative of three independent experiments (** p < 0.01).